(ISO, 2012). It is a high-value cash crop and an exclusive source of 75% of global sugar production (Lakshmanan et al, 2006 and FAO, 2004). This versatile crop is primarily grown for its sweet stem. It also provides many byproducts for biofactories such as alcohol, butanol, acetic acid, plywood, industrial enzymes, animal feed and paper, as well as sugar and energy (Garcia et al, 2007). Undou...... middle of document ......cols for large scale clonal propagation of sugarcane production using apical meristem will be useful for marketing and cultivation. Keeping in mind the scenario described above, this study will be carried out to establish such a protocol for genetically uniform in vitro mass propagation of seedlings from the apical meristem of different sugarcane genotypes, which will ultimately reduce the final cost and seedling production time.2. ObjectivesThe main objective of this study is to develop an optimal protocol for in vitro mass clonal propagation of different sugarcane genotypes from apical meristem culture. The specific objectives are: Optimize the effect of BAP and NAA in combination and alone for shoot multiplication. the effect of NAA for root induction of microshoots. To optimize the effect of table sucrose in the multiplication of shoots

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