ASEPTIC TECHNIQUEAseptic technique is a combination of principles and practices used in experiments to prevent or reduce the possibility of unwanted microorganisms entering cell cultures, sterile solutions, and supplies. This is more serious when working with human cell lines. Good aseptic technique is very important and fundamental to achieving successful results in cell and tissue cultures or any other experiments. When very good aseptic technique is followed, these principles and practices provide many benefits for your cultures, such as protecting the cell line from microbial and cellular cross-contamination, preventing cell line compromise through identification erroneous, protecting the value of your cell line, experiments. and cell culture processes. Here are some aseptic techniques: HYGIENE AND PERSONAL PROTECTION: → Wear a clean gown; For additional protection in the hood, use a cool, closed lab coat with gloves that overlap the wrists. Protective eyewear should be used when appropriate. Lab coats used for cell culture should not leave the cell culture area. → wear clean gloves during aseptic procedures because if we have dry skin and if that skin somehow gets into your experiment your result will be affected. Indeed, dry skin scales are loaded with bacteria. Gloves can also protect you. → be careful when handling sharp equipment to avoid any risk of broken or shattered glass. → Hair should be tied back or covered as hair attracts dirt/dust and can cause contamination. CULTURE AREA: → clean your table or cultivation area before starting; in fact, microorganisms can easily attach to dust particles, so cleaning the table will help us reduce the amount of dust and dirt in the growing area. Also, don't open windows or use window fans that let in outside air during your experiment. If possible, this also to reduce the amount of dirt carried inside. → avoid water baths if possible because wet the outside of a bottle with contaminated water before bringing it. using is never a good idea. → Flame the neck of the bottles you use before and after pouring, as this will prevent contamination from the air bulb entering the tube. → Always use separate media bottles to reduce both potential contaminations; limits the spread of contamination and cross-contamination with another cell line if the media bottle becomes contaminated. → Avoid sharing bottles of medium or other solutions with classmates as this can cause cross-contamination and lack of responsibility also arises from sharing with others.