SUMMARY: Chlamydia trachomatis is one of the most common bacteria found in patients with sexually transmitted infections (STIs), which are almost asymptomatic in the company. Two hundred and sixty urine samples of women divided into two groups (symptomatic and asymptomatic) were collected from patients attending the IST clinic at Mehrad Hospital in Tehran and tested by polymerase chain reaction (PCR) for the presence DNA from C. trachomatis. A total of 39 women in both groups were infected (14.99%), of which 27/130 people were in the symptomatic group (20.76%), compared to 12/130 people in the asymptomatic group (9.23%). . A significant association was observed between C. trachomatis infections and history of STIs in both groups in a dependent manner and with varying urinary parameters such as white blood cells in each group independently, and with epithelial cells only in symptomatic women. The present study shows that screening strategies for Chlamydia infections as part of routine investigations, particularly in symptomatic married women, are INTRODUCTIONS Sexually transmitted infections (STIs) are the second leading cause of unpleasant illness among young people adult women around the world (1). Genital Chlamydia trachomatis infection is one of the most common STIs and is considered the most common treatable and preventable STI and cause of infertility in women (2). Although C. trachomatis infection has been suggested to be a cause of infertility due to the aftereffects on the genital tract, many hospitals and clinics do not routinely screen for C. trachomatis infections. Nowadays, detection of C. trachomatis infections has improved with the development of nucleic acid-based amplification methods such as polymerase chain reaction (PCR) in urine samples and most countries have established...... middle of article ......d that .- In order to optimize this strategy, sexual health services should become widely available and these services should be advertised in a comfortable and attractive in Iran. However, asymptomatic people infected with C. trachomatis play a key role in the spread of infection and may remain in the shadows and therefore continue to spread the infection to their partners. Variable content interventions can lead to promoted changes in information, manners, awareness, self-efficacy, skills and behaviors, such as the use of appropriate procedures for contraceptive methods, causes of sexual infection and practicing safe sex. Finally, further studies to determine the correlations between the infection of people and other variables needed in other cities of Iran. ACKNOWLEDGMENTS This project was supported by Tehran University of Medical Sciences..