A knot was tied about an inch from the end of the dialysis tubes. Then, three graduated cylinders were filled with twenty millimeters of distilled water, 0.5 M sucrose or 1.0 M sucrose. Using a funnel, each solute was poured into the corresponding dialysis tube . To prevent the solute from escaping, another knot was tied about an inch from the opposite end. Once the knot was tied, two fingers were used to remove air bubbles from the bag. Next, each bag was rinsed with distilled water to ensure there was no sucrose residue and then dried with a paper towel. After waiting for a scale to read zero grams, each bag was then rolled onto the scale to identify the mass in grams. The initial mass was recorded. Three containers were brought together and filled about two-thirds with distilled water. Then, the three dialysis tubes were immersed in the corresponding container to soak in distilled water for twenty minutes. To determine the mass of the bags after being soaked in water, the bags were dried with a paper towel and then rolled up again on the scale. The final mass was recorded. Finally, the mass difference and percent mass change were calculated and