Mushrooms are a unique creature in the living world that cannot be classified as either plant or animal. It has become one of the sources of human food. There has been recent discovery of interest in mushrooms not only as a healthy, protein-rich vegetable, but also as a source of biologically active compounds of medical value. Based on Ooi & Liu (2000), schizophyllan from S. commune and lentinan, an interferon-stimulating polysaccharide produced by L. edodes, are products isolated from the fungus to make anticancer drugs. Today, mushroom cultivation has become an agronomy-generating activity that can be applied to small industrial farmers. Therefore, to cultivate the mushroom, there are several steps called medium culture preparation, spawn preparation, medium substrate preparation, spawning, spawn passage, fruiting and harvesting (see Figure 1 in Appendix 1) . The first steps in mushroom cultivation are preparation of culture media. Mushroom mycelium needs nutrition to grow. Agar (seaweed) contains almost no nutrients, but acts as a gelling agent when mixed with water, so that the mycelium has a flat, solid surface to grow on. A combination of agar, water and nutrient is a satisfactory method for developing healthy mycelium (Ogden & Prowse, 2004). The mycelium grows on the surface of the medium and will then be used to inoculate larger quantities of substrates such as cereals. Test tubes or petri dishes used as culture vessels. Young and vigorous mycelium obtained from a young fruiting body of a mushroom. The mycelium should be white and protruding from the tissue. If yellow, blue, green or gray mycelia form elsewhere on the surface, then these are fungal contaminants. A cre...... middle of paper ......ots=psZWcIr-RN&sig=89w1Eyy-xVf_k45DbIqbtyKrbYE#v=onepage&q&f=falseOgden, A. & Prowse, K. (2004). How to spawn oyster mushrooms in a simple way. In R. Gush (Ed.), Mushroom Growers Handbook 1 (pp. 62-74). Seoul, Korea: Mush World. Retrieved from http://www.fungifun.org/mushworld/Oyster-Mushroom-Cultivation/mushroom-growers-handbook-1-mushworld-com-chapter-4-1.pdf Ooi, VEC and Liu, F. (2000). Immunomodulation and anticancer activity of polysaccharide-protein complexes. Current Medicinal Chemistry, 7(7), 715-729. Retrieved from http://www.ingentaconnect.com/content/ben/cmc/2000/00000007/00000007/art00004Shah, ZA, Ashraf, M. and Ishtiaq, CM (2004). Comparative study of the cultivation and yield performance of oyster mushrooms (Pleurotus ostreatus) on different substrates (wheat straw, leaves, sawdust). Pakistani Journal of Nutrition, 3(3), 158-160.